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Rational Drug Design : Methods and Protocols
This volume covers several aspects of rational drug design, such as synthesis of novel bioactive drugs; development and application of new methodologies; computational methods valuable for the establishment of new approaches in drug discovery; and the effects of physical-chemical and ADMET properties of the designed potential drugs.Chapters guide readers through amyloid deposits, Saturation Transfer Difference (STD) NMR, methods on bioguided design, the importance of lipophilicity in drug design, ADMET, FRET, structural biology, and homology modeling.Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Rational Drug Design: Methods and Protocols aims to ensure successful results in the further study of this vital field.
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Rice Protocols
With the completion of a finished rice genome sequence, increasing efforts have focused on functional characterization of rice genes, elucidation of the underlying mechanisms involved in major agronomic traits (e.g., high yield, grain quality, abiotic stress tolerance, and disease resistance), and the subsequent translation of genomic knowledge into agricultural productivity via molecular breeding and improved cultural practice.To meet increasing interest in this field, Rice Protocols has been compiled to provide a series of core techniques and approaches commonly used in studying rice molecular biology and functional genomics.These approaches include genetic and molecular techniques such as artificial hybridization, fluorescence in situ hybridization, generation and characterization of chemical and T-DNA insertional mutants, quantitative trait loci (QTLs) analysis and map-based cloning, site-specific transgene integration, and artificial microRNA-mediated gene silencing, along with a variety of “omics” techniques. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and easy to use, Rice Protocols will prove useful for both beginners and experienced researchers whether they are molecular biologists who want to study rice plants or rice researchers who are interested in learning molecular techniques.
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On Call Principles and Protocols : Principles and Protocols
Ideal for any on-call professional, resident, or medical student, this highly templated, best-selling reference covers the common problems you’ll encounter while on call in the hospital.On Call Principles and Protocols, 7th Edition, by Drs.Shane A. Marshall and John Ruedy, provides key information in time-sensitive, challenging situations.You’ll gain speed, skill, and knowledge with every call - from diagnosing a difficult or life-threatening situation to prescribing the right medication. Features a logical, highly templated format so you can locate critical information quickly. Includes new content on COVID-19 in chapters on Viruses and Shortness of Breath and Fever. Covers essential topics such as Approach to Diagnosis and Management of On-Call Problems; Documentation; Assessment and Management of Volume Status; and HIV, HBV, HCV, Influenza, and the House Officer. Delivers consistent, easy-to-follow coverage of the most common on-call problems and approaches, including what to do from the initial phone call, "Elevator Thoughts," how to immediately identify major threats to life, what to do at the bedside, and how to avoid common mistakes for every call. Provides updated content and references, as well as an extensively revised drug formulary of commonly prescribed medications, keeping you on the cutting edge of current, evidence-based information. Serves as the perfect bridge to clinical medicine for students transitioning to the hospital setting, a trusty "peripheral brain" for residents, and a handy reminder for more experienced practitioners. An eBook version is included with purchase. The eBook allows you to access all of the text, figures, and references, with the ability to search, customize your content, make notes and highlights, and have content read aloud.
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Attacking Network Protocols
Attacking Network Protocols is a deep-dive into network vulnerability discovery from James Forshaw, Microsoft's top bug hunter.This comprehensive guide looks at networking from an attacker's perspective to help you find, exploit, and ultimately protect vulnerabilities.Part I starts with a rundown of networking basics and traffic capture, as it builds a foundation for analyzing a network.Part II moves on to protocol analysis, both static and dynamic; you'll learn about common protocol structures, cryptography, and protocol security, and how to reverse engineer code with IDA Pro, ILSpy, and Javasnoop.Part III focuses on finding and exploiting vulnerabilities, including an overview of common bug classes, fuzzing, debugging, exhaustion attacks, and how to develop custom tools.Forshaw ends with an overview of the best tools for analyzing and exploiting networks.By the book's end, you'll have a deep understanding of how to analyze network communication and where to look for vulnerabilities.You'll learn how to--Capture, manipulate, and spoof packets both passively and on the wire-Reverse engineer code, brute force passwords, and decrypt traffic-Exploit vulnerabilities with denial-of-service attacks, authentication and authorization bypasses, and memory corruptions-Use capture and analysis tools like IDA Pro, Wireshark, and CANAPE-Strengthen your exploits by rerouting network traffic, exploiting compression, and controlling data flowAttacking Network Protocols is a must-have for any penetration tester, bug hunter, or developer looking to exploit and secure network vulnerabilities.
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Tinnitus Treatment : Clinical Protocols
The quintessential clinical guide for audiologists on tinnitus and hyperacusis patient managementSince publication of the first edition in 2005, new developments have impacted the treatment paradigm for tinnitus, such as sensory meditation and mindfulness.Tinnitus Treatment: Clinical Protocols, Second Edition, by world-renowned tinnitus experts and distinguished authors Richard S.Tyler and Ann Perreau provides comprehensive background information, up-to-date strategies, essential tools, and online supplementary materials grounded in years of clinical experience and research.It fills a gap in graduate education and available materials to empower audiologists to effectively treat patients suffering from bothersome to severely debilitating symptoms associated with tinnitus or hyperacusis. The textbook includes 15 chapters, starting with three chapters on tinnitus models, treatment approaches, and self-treatment options.The next three chapters summarize counseling approaches for audiologists and psychologists, including introduction of the three-track psychological counseling program for managing tinnitus.Chapters 7 and 8 discuss the use of hearing aids in patients with hearing loss-related tinnitus and sound therapy using wearable devices.Chapter 9 covers smartphone apps for tinnitus assessment, management, and education and wellness, including discussion of limitations.The last six chapters provide guidance on tinnitus management topics including treating children, implementing outcome measures, hyperacusis treatment, and future directions. Key FeaturesNew relaxation/distraction tactics including meditation, mindfulness, guided imagery, biofeedback, progressive muscle relaxation, art and music therapy, exercise, and exploration of new hobbiesTreatment guidance for patients with tinnitus associated with Meniere's disease, vestibular schwannoma, unilateral sudden sensorineural hearing loss, and middle ear myoclonusDiscussion and research-based evidence covering Internet-delivered self-help treatment strategiesNew supplemental videos, brochures, handouts, questionnaires, and datasheets enhance knowledge, scope of practice, and incorporation of effective approaches into clinical practiceThis is a must-have resource for every audiology student and advanced courses, as well as essential reading for all audiologists who feel underprepared in managing tinnitus and/or hyperacusis. This book includes complimentary access to a digital copy on https://medone.thieme.com.
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Drosophila : Methods and Protocols
This third edition expands on the previous editions by providing updated protocols and current methods that cover recent breakthroughs in Drosophila research.Chapters guide readers through FlyBase, versatile gene expression systems, analysis of microRNA function, single-cell transcriptome data and metabolism, recent applications of CRISPR for precise genome editing, transcriptional activation and cell lineage tracing, protein inhibition and tagging, optogenetic and optochemical control of tissue mechanics, AFM measurements, sample preparation for electron microscopy, live imaging of different tissues and organs, and quantitative image analysis.Written in the format of the highly successful Methods in Molecular Biology series, chapters include an introduction to their topic, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, as well as tips on troubleshooting and avoiding known pitfalls Authoritative and cutting-edge, Drosophila: Methods and Protocols, Third Edition serves as a useful and practical guide to new researchers and experts using Drosophila as a model system. Chapter 7 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
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Immunoproteomics : Methods and Protocols
This second edition volume expands on the previous edition with new sections describing the characterization of peptides bound to major histocompatibility complexes (MHC) on the surface of the cell.Chapters also cover topics such as using SERPA for antigen identification; antigen content of electroimmunoprecipitates; whole genome-phage display libraries; antigens in immune complexes; and immunoproteomic biomarkers.Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Immunoprotemics: Methods and Protocols, Second Edition is a valuable resource that presents novice and expert researchers with techniques that are easily transferrable to laboratories and provides enhancedhands-on insights into this evolving field.
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Protocols for Macroalgae Research
This book presents a wide range of tested and proven protocols relevant to a number of fields within biotechnology used in laboratory experiments in everyday phycological (seaweed) research.A major focus will be on bioenergy related aspects of this emerging technology.These protocols will be written in a clear and concise manner using simple language permitting even nonspecialist to adequately understand the significance of this research.It will also contain all necesssary notes and guidelines for successful execution of these experiments.
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